Preovulatory changes in morphology of rabbit ovarian follicles.

Preovulatory changes in morphology of rabbit ovarian follicles.

To examine timed changes in the morphology of theca interna and granulosa cells of rabbit preovulatory follicles, mature female rabbits were injected with100 IU per kg of human chorionic gonadotropin (hCG) and follicles larger than1mn in diameter were excised for light and electron microscopic studies before and3, 6, 9and 12hr after hCG injection. Spindle-shaped theca interna cells had been tra...

Progestagen dynamics in preovulatory follicles of rats.

Selective activation of rabbit ovarian protein kinase isozymes in rabbit ovarian follicles and corpora lutea.

The magnitude of activation of the type I and type II forms of cAMP-dependent protein kinase was investigated in estrous follicles and corpora lutea (CL) obtained from ovaries of control rabbits and rabbits injected acutely with human chorionic gonadotropin (hCG). To this end, a chromatographic technique which permitted quantitative evaluation of the in vivo activational state of the two forms ...

Programmed cell death in preovulatory ovine follicles.

Apoptosis is a mode of physiological cell death that occurs during tissue regression and remodeling; it is characterized by macromolecular protein synthesis, oligonucleosomal fragmentation, nuclear condensation, cytoplasmic shrinkage, and dissociation of cell-to-cell junctional complexes. Direct in situ fluorescence detection of digoxigenin end-labeled genomic DNA was used as a marker of nuclea...

Rhythmic expression of circadian clock genes in the preovulatory ovarian follicles of the laying hen

The circadian clock is reported to play a role in the ovaries in a variety of vertebrate species, including the domestic hen. However, the ovary is an organ that changes daily, and the laying hen maintains a strict follicular hierarchy. The aim of this study was to examine the spatial-temporal expression of several known canonical clock genes in the granulosa and theca layers of six hierarchy f...